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Single molecule array (Simoa™)- 給你femtogram靈敏度的ELISA

2015/11/11
Single molecule array (Simoa™) 為超靈敏ELISA技術,有別於傳統sandwich ELISA picogram等級,Simoa技術提供Femto等級的超高靈敏度,幫助你更能掌握微量蛋白的變化。


 
實驗原理
Simoa跟傳統ELISA一樣都是sandwich的方式,差別在於Simoa可以偵測每一個在beads上的抗原抗體複合物,偵測”digital “訊號,只要有一個分子有接上抗原即可被偵測到,因此較傳統ELISA靈敏

 


其他數據

 
Reference
  • Olympic boxing is associated with elevated levels of the neuronal protein tau in plasma
    March 8, 2013
    Brain Injury
    Neselius S, Zetterberg H, Blennow K, Randall J, Wilson D, Marcusson J, Brisby H.
  • Single molecule enzyme-linked immunosorbent assays: Theoretical considerations
    Lei Chang, David M. Rissin, David R. Fournier, Tomasz Piech, Purvish P. Patel, David H. Wilson, David C. Duffy. Journal of Immunological Methods 378 (2012) pp. 102-115
  • Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations.
    David M Rissin, Cheuk W Kan, Todd G Campbell, Stuart C Howes, David R Fournier, Linan Song, Tomasz Piech, Purvish P Patel, Lei Chang, Andrew J Rivnak, Evan P Ferrell, Jeffrey D Randall, Gail K Provuncher, David R Walt & David C Duffy
    Nature Biotechnology 28, 595–599 (May 2010) doi:10.1038/nbt.164
   

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