產品及服務

蛋白質定量

CB™ Protein Assay

蛋白質定量試劑-Bradford 方式

產品說明
為Bradford方法學。為最簡便的蛋白質定量方法
Protocol (289 KB)
功能說明
Coomassie Dye會與蛋白質結合,其顏色會從棕色變成藍色,利用其顏色的變化,於OD.595nm進行偵測
規格說明
貨號 品名 包裝
786-012 CB™ Protein Assay
(With BSA Protein Standard)
500 assays
786-893 CB™ Protein Assay
(With Non-Animal Protein Standard)
500 assays
其它說明1
Table 1 : A selection of compounds and the maximum concentrations that are compatible with the CB™ Protein Assay
Compound Concentration Compound Concentration Compound Concentration
Amino acid 1mM DTT 1M Phenol 5%
Ammonium sulfate 1M EDTA 100mM Sodium azide 0.5%
Ampholytes 0.5% EGTA 50mM Sodium chloride 6M
Ascorbic acid 50mM Ethanol 10% Sodium dodecyl sulfate (SDS) 0.015%
Boric acid 1mM Glucose 1M Sodium hydroxide 0.1M
Brij 35 0.06% Glycerol 10% Sodium phosphate 0,1M
CHAPS 0.5% Glycine 0.1M Sucrose 25%
CHAPSO 0.5% Guanidine.HCl 6M Tris 2M
Citrate 0.05% HEPES 0.1M Triton X-100 0.06%
Cysteine 10nM 2-mercaptoethanol 1M tRNA 0.35mg/ml
Deoxycholate 0.1% Methanol 10% Tween 20 0.03%
DMSO 10% MES 0.7M Urea 3M
DNA 1mg/ml Nonidet P-40 0.5%








 
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