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Blotting Kits and reagents 墨點法相關試劑
Western Blotting;HRP;AP;TMB;ECL;KPL;wash buffer

Blotting Substrates and Reagents

西方墨點法呈色劑與相關試劑

功能說明
       免疫分析中Substrates是detection和呈色步驟最重要的決定因子;如何選擇適當的Substrates,則可依據對敏感度的需求、不同的儀器與濾鏡求、以及酵素的選擇。酵素系統主要為HRP和AP系統。。HRP的酵素反應催化時間比AP來的快,在短時間內可以反應較多的受質,因此短時間內的敏感度很高;AP反應的優點之一,就是AP與其受質(substrates)的反應呈現一線性反應,所以其敏感度可隨著受質反應時間延長而提升。研究者可依其需求來選擇適當的substrates。SeraCare KPL發展出專門適合於PVDF和NC membrane使用的液狀化學呈色與冷光呈色試劑;具有方便、穩定、濃度範圍廣泛與敏感度高等特性。


 
HRP Membrane Substrates
Cat. # Product Name Size
5420-0020
(50-73-00)
4CN 2-Component Membrane Peroxidase Substrate Kit 600 mL
5420-0024
(50-73-04)
4CN 2-Component Membrane Peroxidase Substrate Kit 2.7 L
5430-0040
(54-61-00)
LumiGLO Peroxidase Chemiluminescent Substrate Kit 240 mL
5430-0041
(54-61-01)
LumiGLO Peroxidase Chemiluminescent Substrate Kit 720 mL
5430-0042
(54-61-02)
LumiGLO Peroxidase Chemiluminescent Substrate Kit 60 mL
5430-0049
(54-71-00)
LumiGLO Reserve Chemiluminescent Substrate Kit 2400 cm2
5430-0050
(54-71-01)
LumiGLO Reserve Chemiluminescent Substrate Kit 600 cm2
5420-0027
(50-77-03)
TMB 1-Component Membrane Peroxidase Substrate 200 mL
5420-0028
(50-77-04)
TMB 1-Component Membrane Peroxidase Substrate 1 L
5420-0029
(50-77-18)
TMB 1-Component Membrane Peroxidase Substrate 100 mL
5420-0026
(50-77-01)
TMB Membrane Enhancer 40 mL
5420-0025
(50-77-00)
TMB Membrane Peroxidase Substrate System 440 mL
AP Membrane Substrates
Cat. # Product Name Size
5420-0033
(50-81-07)
BCIP/NBT 1-Component Phosphatase Substrate 600 mL
5420-0036
(50-81-10) 
BCIP/NBT 1-Component Phosphatase Substrate 1 L
5420-0038
(50-81-18)
BCIP/NBT 1-Component Phosphatase Substrate 100 mL
5420-0030
(50-81-00)
BCIP/NBT Phosphatase Substrate System 300 mL
5430-0054
(55-60-03)
PhosphaGLO AP Substrate 30 mL
5430-0055
(55-60-04)
PhosphaGLO AP Substrate 100 mL
5430-0052
(55-60-01)
PhosphaGLO Reserve AP Substrate 30 mL
5430-0053
(55-60-02)
PhosphaGLO Reserve AP Substrate 100 mL

另外,岑祥也提供您其他在Blotting時可使用的試劑;包括 blocking, and washing steps of the assay, and antibody conjugate stabilizers and substrate stop solutions:

 
Cat. # Product Name Size
5960-0021
(50-86-05)
20X SSC 1 L
5960-0015
(50-63-06)
Biotin Wash Kit 200 mL
5150-0008
(50-63-00)
Wash Solution Concentrate Kit 800 mL
5140-0006
(50-61-00)
10% BSA Diluent/Blocking Solution 200 mL
5140-0008
(50-61-10)
10% BSA Diluent/Blocking Solution 1 L
5560-0007
(71-00-27)
10% Normal Goat Serum 50 mL
5560-0010
(71-18-01)
10% Normal Mouse Serum 10 mL
5560-0008
(71-00-28)
10% Normal Rabbit Serum 50 mL
5920-0004
(71-83-00)
5X Detector Block 240 mL
5560-0006
(71-00-10)
Blocking Solution Concentrate 10 mL
5140-0011
(50-82-01)
Milk Diluent/Blocking Concentrate Kit 200 mL
5560-0009
(71-00-61)
Universal Block for Immunohistochemistry 100 mL
5290-0007
(55-15-00)
AP Stabilizer 200 mL
5290-0005
(54-15-01)
HRP Stabilizer 200 mL
5120-0058
(50-80-02)
DEA Buffer Solution 100 mL
5570-0005
(71-00-16)
Fluorescent Mounting Media 15 mL
5450-0012
(50-22-02)
Silver Enhancer Kit for Membranes 500 mL
5520-0021
(50-22-01)
Silver Enhancer Kit for Microscopy 50 mL
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